Coding

Part:BBa_K3392000:Design

Designed by: Xian-en Zhang   Group: iGEM20_UCAS-China   (2020-10-23)


Doc-SsrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (TEV site) can be replaced by other suitable sites.


Source

Gottesman, S. , Roche, E. , Zhou, Y. , & Sauer, R. T. . (1998). The clpxp and clpap proteases degrade proteins with carboxy-terminal peptide tails added by the ssra-tagging system. Genes Dev, 12(9), 1338-1347.

References